价格：570  、 200

    货号：03-053-1A、 03-053-1B

    规格：500ml   、100ml

    产品名称：Trypsin EDTA Solution C (0.05%), EDTA (0.02%), 

               with Phenol Red

               胰酶（胰蛋白酶0.05%，EDTA0.02%）

【产品介绍】

 Trypsin, an animal-derived product, is the most commonly used  enzyme for harvesting cells

 in culture. Trypsin is a pancreatic serine   protease (proteolytic) enzyme with specificity for peptide

 bonds involving the carboxyl group of the basic amino acids, Arginine and Lysine. Trypsin often 

contains a crude mixture of lipases, nucleases, polysaccharides and proteases extracted from Porcine pancreas.

Most cell cultures grow as a single thickness cell layer or sheet attached to a substrate known 

as a monolayer. When subculturing adherent cells, these intercellular and cell-to-substrate links

 or connections must be gently dissociated. Proteolytic enzymes such as Trypsin (i.e. a serine 

peptidase), breaks or gently separates these bonds by creating a single-cell suspension from

 which new subcultures are realized. Trypsin solutions are widely utilized as cell dissociation

 reagents for continuous cell culture of adherent growing cells. Trypsin proteolysis or trypsinization

 is a process in which proteins have been digested or treated with Trypsin and are thus said to be trypsinized. 

Biological Industries' Trypsin is designed not only to gently dissociate cells from almost any 

support substrates but also as well as from each other in order to actualize cell manipulation

 techniques in addition for other studies that require intact cell-surface proteins. Trypsin, as a 

solution, is available in a varied array of  ormulations with or without EDTA. EDTA is a chelator 

that binds calcium and magnesium ions that may otherwise inhibit the Trypsin activity which 

then hydrolyzes and gains access to the intercellular bonds being cell-cell and/or cell-substrate 

bonds. Crude Trypsin is often the subculturing agent of choice for cell dissociation/disaggregation 

of adherent cells although the  treatment may be cytotoxic if prolonged. Over-trypsinization is 

a common cause of subculture problems.

The use of Crude Trypsin often involves multiple changes and the variability among lots can

 dramatically influence the effectiveness of the dissociation process. Regarding the use of Crude

 Trypsin, some important facts must be noted:

v Cells must NEVER remain in the Crude Trypsin for longer than 3-5 minutes as they may be

 seriously damaged in the process (i.e. damage to the intracellular proteins).

v Cells should NEVER be left without a fluid layer

v Do not permit prolonged growth on culture ware as the cells will be very difficult to remove(i.e. after5-7 days)

In a serum-free culture environment, the cells must be separated by rapid centrifugation or 

by utilizing Trypsin inhibitors such as Soybean Trypsin Inhibitor (SBTI). SBTI is a single polypeptide 

that forms a stable, stoichiometric, enzymically inactive complex with Trypsin thereby reducing 

the availability of Trypsin by, more or less, binding chymotrypsin. However, with Biological Industries' 

Soybean Trypsin Inhibitor, any excess Crystalline Trypsin may be completely neutralized, thereby 

avoiding the use of serum for this purpose. The cells may then be resuspended successfully in a 

suitable growth medium.

Predominant Characteristics of Trypsin EDTA Solution B include:

Predominant Characteristics of Trypsin EDTA Solution C (0.05%), EDTA (0.02), with Phenol Red include:

§ Animal-Derived Source

§ Trysin(0.05%);EDTA(0.02%)

§ Meets USP and EPTesting Specifications

§ Cell-Culture-Tested

§ Suitable for Cell-Culture Applications

§ Long-Storage When Handled Properly Under Defined Conditions

说明书下载：

6381101590432004103527373.pdf