价格：350

    货号：03-046-5B

    规格：100ml

    产品名称：Trypsin Solution B (2.5%), 10X Conc., without Calcium 

              and Magnesium, without Phenol Red

              胰蛋白酶（不含钙和镁，不含酚红，10倍浓缩液）

【产品介绍】

Trypsin, an animal-derived product, is the most commonly used enzyme for harvesting cells in 

culture. Trypsin is a pancreatic serine protease (proteolytic) enzyme with specificity for peptide 

bonds involving the carboxyl group of the basic amino acids, Arginine and Lysine. Trypsin often

 contains a crude mixture of lipases, nucleases, polysaccharides and proteases extracted from 

Porcine pancreas.

Most cell cultures grow as a single thickness cell layer or sheet attached to a substrate known

 as a monolayer. When subculturing adherent cells, these intercellular and cell-to-substrate 

links or connections must be gently dissociated. Proteolytic enzymes such as Trypsin (i.e. a serine

 peptidase), breaks or gently separates these bonds by creating a single-cell suspension from

 which new subcultures are realized. Trypsin solutions are widely utilized as cell dissociation 

reagents for continuous cell culture of adherent growing cells. Trypsin proteolysis or trypsinization

 is a process in which proteins have been digested or treated with Trypsin and are thus said to

be trypsinized. Biological Industries' Trypsin is designed not only to gently dissociate cells from 

almost any support substrates but also as well as from each other in order to actualize cell 

manipulation techniques in addition for other studies that require intact cell-surface proteins. 

Trypsin, as a solution, is available in a varied array of  ormulations with or without EDTA. EDTA

 is a chelator that binds calcium and magnesium ions that may otherwise inhibit the Trypsin activity

 which then hydrolyzes and gains access to the intercellular bonds being cell-cell and/or cell-substrate

 bonds. Crude Trypsin is often the subculturing agent of choice for cell dissociation/disaggregation

 of adherent cells although the  treatment may be cytotoxic if prolonged. Over-trypsinization is

 a common cause of subculture problems.

The use of Crude Trypsin often involves multiple changes and the variability among lots can 

dramatically influence the effectiveness of the dissociation process. Regarding the use of Crude 

Trypsin, some important facts must be noted:

v Cells must NEVER remain in the Crude Trypsin for longer than 3-5 minutes as they may be 

seriously damaged in the process (i.e. damage to the intracellular proteins).

v Cells should NEVER be left without a fluid layer

v Do not permit prolonged growth on culture ware as the cells will be very difficult to 

remove(i.e. after5-7 days)

In a serum-free culture environment, the cells must be separated by rapid centrifugation or by 

utilizing Trypsin inhibitors such as Soybean Trypsin Inhibitor (SBTI). SBTI is a single polypeptide 

that forms a stable, stoichiometric, enzymically inactive complex with Trypsin thereby reducing

 the availability of Trypsin by, more or less, binding chymotrypsin. However, with Biological 

Industries' Soybean Trypsin Inhibitor, any excess Crystalline Trypsin may be completely neutralized,

 thereby avoiding the use of serum for this purpose. The cells may then be resuspended successfully 

in a suitable growth medium.

Predominant Characteristics of Trypsin Solution B (0.25% in DPBS) without Calcium & Magnesium,

 without Phenol Red include:

§ Animal-Derived Source

§ Meets USP and EPTesting Specifications

§ Cell-Culture-Tested

§ Suitable for Cell-Culture Applications

§ Long-Storage When Handled Properly Under Defined Conditions

说明书下载：

6381101566233924104044976.pdf