价格：160

    货号：C3011-0500

    规格：500ml

    产品名称：超低内毒素RPMI1640（含谷氨酰胺，SLE级别）RPMI 1640 With L-Glutamine, SLE Grade

【产品介绍】     

Roswell Park Memorial Institute (RPMI) media are a series of media developed by Moore 

et al for the culture of human normal and neoplastic cells in vitro. RPMI 1640 is the most 

commonly used medium in the series. A modification of McCoy's 5A medium, the medium 

was specifically designed to support the growth of human lymphoblastoid cells in suspension 

culture.  Presently the medium is extensively used for a wide range of anchorage dependant 

cell lines. The medium needs to be supplemented with 5-20% fetal bovine serum. The medium 

is also known to support growth of cells in the absence of serum.

It is super low endotoxin SLETM grade，Endotoxin≤0.01EU/ml by LAL method. The production

 process and quality management system meet the requirements of cGMP and has passed 

ISO13485 certification.

Users are advised to review the literature for recommendations regarding medium 

supplementation and physiological growth requirements specific for different cell lines.                                       

【Storage and Stability】

RPMI 1640 , with L-Glutamine should be kept 2-8°C. The product is light -sensitive and therefore 

should not be left in the light. When stored in the dark under ideal conditions, the product is 

stable until the expiry date.

As with any other liquid media formulations, deterioration of  liquid media may be recognized 

by any of the following characteristics, among others including:

(a). Color Change;

(b). Presence of clumping/flocculent debris/ granulation/ particulates\ precipitates or sediments ;

(c). Insolubility;

(d). And/or decrease in expected performance parameters.

Any material described above should not be used and therefore discarded.

【Procedure】

1.Take a bottle from the defined storage conditions at 2-8°C and read the label.

2.Wipe the outside of the bottle with a disinfectant solution such as 70% ethanol.

3.Using aseptic/sterile technique under a laminar-flow culture hood, work according 

to established protocols.

4.Antibiotics may be added if desired.

【Quality Contro】

RPMI 1640, with L-Glutamine is tested for sterility, pH, osmolality and endotoxin concentrations.

 In addition, each batch is tested for cell growth using Vero.

【Precaution and Disclaimer】

For research use only, not for clinical diagnosis and treatment.

说明书下载：

6380678554913145106737882.pdf